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. 1991 Jun:176:105-12.

Autoradiographic analysis of vasoactive neuropeptide uptake by rabbit megakaryocytes and platelets

T Daimon  1
Affiliations

Autoradiographic analysis of vasoactive neuropeptide uptake by rabbit megakaryocytes and platelets

T Daimon. J Anat. 1991 Jun.

Abstract

Autoradiographic analysis has been performed to determine whether or not platelets and megakaryocytes can take up in vitro vasoactive neuropeptides such as [3H]angiotensin II, [3H]neuropeptide Y, [3H]substance P and [3H]vasopressin. Light microscope autoradiography has revealed the active uptake of [3H]angiotensin II by megakaryocytes. [3H]angiotensin II uptake by platelets has also been confirmed by electron microscope autoradiography. Silver grains are preferentially associated with the dense bodies of platelets. Neither megakaryocytes nor platelets show any active uptake of [3H]neuropeptide Y or [3H]substance P. Megakaryocytes are slightly labelled after incubation with [3H]vasopressin.

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