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Comparative Study
. 2006 Dec 15;127(6):1223-37.
doi: 10.1016/j.cell.2006.10.037.

Reconstitution, activities, and structure of the eukaryotic RNA exosome

Quansheng Liu  1 , Jaclyn C Greimann, Christopher D Lima
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Free article
Comparative Study

Reconstitution, activities, and structure of the eukaryotic RNA exosome

Quansheng Liu et al. Cell. .
Free article

Erratum in

  • Cell. 2007 Oct 5;131(1):188-9

Abstract

The RNA exosome is a multisubunit 3' to 5' exoribonuclease complex that participates in degradation and processing of cellular RNA. To determine the activities and structure of the eukaryotic exosome, we report the reconstitution of 9-subunit exosomes from yeast and human and reconstitution of 10- and 11-subunit exosomes from yeast. Comparative biochemical analysis between purified subunits and reconstituted exosomes using AU-rich, polyadenylated (poly[A]), generic, and structured RNA substrates reveals processive phosphorolytic activities for human Rrp41/Rrp45 and the 9-subunit human exosome, processive hydrolytic activities for yeast Rrp44 and the yeast 10-subunit exosome, distributive hydrolytic activities for Rrp6, and processive and distributive hydrolytic activities for the yeast 11-subunit exosome. To elucidate the architecture of a eukaryotic exosome, its conserved surfaces, and the structural basis for RNA decay, we report the X-ray structure determination for the 286 kDa nine-subunit human exosome at 3.35 A.

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