Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Jan;24(1):17-22.
doi: 10.1007/s10815-006-9071-z. Epub 2006 Dec 16.

Insights on blastomere nuclearity

Mónica Gil  1 , Gustavo D'Ommar, Maria E Póo, Anna Sosa, Marta Piras, Romano Piras, Francisco Rísquez
Affiliations

Insights on blastomere nuclearity

Mónica Gil et al. J Assist Reprod Genet. 2007 Jan.

Abstract

Purpose: To analyze the results of our transferred embryos, especially those that "changed" their blastomere nuclearity from Multinucleated (MN) to Mono-nucleated during development.

Methods: Pregnancies where at least one MN embryo was transferred were retrospectively evaluated and categorized in order to record and follow-up on the ones that were implanted. Embryos were classified as normal (when all blastomeres were mono-nucleated on day one and two of development), corrected (multinucleated embryos on day one that became mono-nucleated on day two) and non-corrected (multinucleated either on day one, on day two or both days).

Results: There were 633 transfer cycles analyzed. Thirty-three percent (206) had at least one embryo with a MN blastomere at a given stage of development. Pregnancy and implantation rates were 29.0% and 19.0% for the group of exclusively mono-nucleated embryo transfers, and 28.6% and 15.8% for the group with at least one MN embryo transferred. The pregnancy outcome for "corrected" and "non-corrected" embryos could be corroborated unequivocally in only 9 cases, with an outcome of 8 and 4 normal babies, respectively.

Conclusions: Because the amount of data analyzed is not satisfactorily large, differences were not significantly different; however, a trend may exist showing that normal at term pregnancies obtained from corrected embryos are more likely to occur than those from non-corrected embryos. Nuclear observation on a daily basis should be one of the strategies used to select the best embryos for transferring, to improve implantation rates and avoid multiple pregnancies.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Fig. 2
Fig. 2
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Hardy K, Winston RM, Handyside AH. Binucleate blastomeres in preimplantation human embryos in vitro: Failure of cytokinesis during early cleavage. J Reprod Fertil. 1993;98(2):549–558. doi: 10.1530/jrf.0.0980549. - DOI - PubMed
    1. Jackson KV, Ginsburg ES, Hornstein MD, Rein MS, Clarke RN. Multinucleation in normally fertilized embryos is associated with an accelerated ovulation induction response and lower implantation and pregnancy rates in in vitro fertilization-embryo transfer cycles. Fertil Steril. 1998;70(1):60–66. doi: 10.1016/S0015-0282(98)00100-9. - DOI - PubMed
    1. Van Royen E, Mangelschots K, Vercruyssen M, De Neubourg D, Valkenburg M, Ryckaert G, Gerris J. Multinucleation in cleavage stage embryos. Hum Reprod. 2003;18(5):1062–1069. doi: 10.1093/humrep/deg201. - DOI - PubMed
    1. Moriwaki T, Suganuma N, Hayakawa M, Hibi H, Katsumata Y, Oguchi H, Furuhashi M. Embryo evaluation by analysing blastomere nuclei. Hum Reprod. 2004;19(1):152–156. doi: 10.1093/humrep/deh003. - DOI - PubMed
    1. Meriano J, Clark C, Cadesky K, Laskin CA. Binucleated and micronucleated blastomeres in embryos derived from human assisted reproduction cycles. Reprod Biomed Online. 2004;9(5):511–520. - PubMed