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. 2006 Sep-Oct;52(5):458-68.

[Nanoelectrochemistry of cytochrome P450s: direct electron transfer and electrocatalysis]

[Article in Russian]
V V Shumiantseva, T V Bulko, Iu O Rudakov, N F Samenkova, A V Lisitsa, I I Karuzina, A I Archakov
  • PMID: 17180920

[Nanoelectrochemistry of cytochrome P450s: direct electron transfer and electrocatalysis]

[Article in Russian]
V V Shumiantseva et al. Biomed Khim. 2006 Sep-Oct.

Abstract

The present study demonstrates the direct electron transfer between cytochrome P450 2B4 (CYP2B4), P450 1A2 (CYP1A2), sterol 14alpha-demethylase (CYP51MT) and screen printed graphite electrodes, modified with gold nanoparticles and didodecyldimethylammonium bromide (DDAB). Electrodetection of heme proteins is possible when 2-200 pmol P450/electrode were adsorbed on the surface of nanostructured electrochemical interfaces. Electron transfer, direct electrochemical reduction and interaction with P450 substrates (oxygen, benzphetamine, lanosterol) and inhibitor ketoconazole were analyzed using cyclic voltammetry (CV), square wave (SWV) or differential pulse (DPV) voltammetry, amperometry.

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