Polysaccharide intercellular adhesin or protein factors in biofilm accumulation of Staphylococcus epidermidis and Staphylococcus aureus isolated from prosthetic hip and knee joint infections
- PMID: 17187854
- DOI: 10.1016/j.biomaterials.2006.11.046
Polysaccharide intercellular adhesin or protein factors in biofilm accumulation of Staphylococcus epidermidis and Staphylococcus aureus isolated from prosthetic hip and knee joint infections
Abstract
Nosocomial staphylococcal foreign-body infections related to biofilm formation are a serious threat, demanding new therapeutic and preventive strategies. As the use of biofilm-associated factors as vaccines is critically restricted by their prevalence in natural staphylococcal populations we studied the distribution of genes involved in biofilm formation, the biofilm phenotype and production of polysaccharide intercellular adhesin (PIA) in clonally independent Staphylococcus aureus and Staphylococcus epidermidis strains isolated from prosthetic joint infections after total hip or total knee arthroplasty. Biofilm formation was detected in all S. aureus and 69.2% of S. epidermidis strains. Importantly, 27% of biofilm-positive S. epidermidis produced PIA-independent biofilms, in part mediated by the accumulation associated protein (Aap). Protein-dependent biofilms were exclusively found in S. epidermidis strains from total hip arthroplasty (THA). In S. aureus PIA and proteins act cooperatively in biofilm formation regardless of the infection site. PIA and protein factors like Aap are of differential importance for the pathogenesis of S. epidermidis in prosthetic joint infections (PJI) after THA and total knee arthroplasty (TKA), implicating that icaADBC cannot serve as a general virulence marker in this species. In S. aureus biofilm formation proteins are of overall importance and future work should focus on the identification of functionally active molecules.
Similar articles
-
Biofilm formation, icaADBC transcription, and polysaccharide intercellular adhesin synthesis by staphylococci in a device-related infection model.Infect Immun. 2005 Mar;73(3):1811-9. doi: 10.1128/IAI.73.3.1811-1819.2005. Infect Immun. 2005. PMID: 15731082 Free PMC article.
-
Does Extracellular DNA Production Vary in Staphylococcal Biofilms Isolated From Infected Implants versus Controls?Clin Orthop Relat Res. 2017 Aug;475(8):2105-2113. doi: 10.1007/s11999-017-5266-0. Epub 2017 Feb 13. Clin Orthop Relat Res. 2017. PMID: 28194715 Free PMC article.
-
Host factors abolish the need for polysaccharides and extracellular matrix-binding protein in Staphylococcus epidermidis biofilm formation.J Med Microbiol. 2021 Mar;70(3):001287. doi: 10.1099/jmm.0.001287. Epub 2021 Jan 22. J Med Microbiol. 2021. PMID: 33492206 Free PMC article.
-
Structure, function and contribution of polysaccharide intercellular adhesin (PIA) to Staphylococcus epidermidis biofilm formation and pathogenesis of biomaterial-associated infections.Eur J Cell Biol. 2010 Jan;89(1):103-11. doi: 10.1016/j.ejcb.2009.10.005. Epub 2009 Nov 14. Eur J Cell Biol. 2010. PMID: 19913940 Review.
-
Polysaccharide intercellular adhesin in biofilm: structural and regulatory aspects.Front Cell Infect Microbiol. 2015 Feb 10;5:7. doi: 10.3389/fcimb.2015.00007. eCollection 2015. Front Cell Infect Microbiol. 2015. PMID: 25713785 Free PMC article. Review.
Cited by
-
Susceptibility patterns of Staphylococcus aureus biofilms in diabetic foot infections.BMC Microbiol. 2016 Jun 23;16(1):119. doi: 10.1186/s12866-016-0737-0. BMC Microbiol. 2016. PMID: 27339028 Free PMC article.
-
The Role of Staphylococcal Biofilm on the Surface of Implants in Orthopedic Infection.Microorganisms. 2022 Sep 26;10(10):1909. doi: 10.3390/microorganisms10101909. Microorganisms. 2022. PMID: 36296183 Free PMC article. Review.
-
The staphylococcal biofilm protein Aap forms a tetrameric species as a necessary intermediate before amyloidogenesis.J Biol Chem. 2020 Sep 11;295(37):12840-12850. doi: 10.1074/jbc.RA120.013936. Epub 2020 Jul 14. J Biol Chem. 2020. PMID: 32665400 Free PMC article.
-
Prevalence of Virulence-Related Determinants in Clinical Isolates of Staphylococcus epidermidis.Jundishapur J Microbiol. 2016 Feb 13;9(8):e30593. doi: 10.5812/jjm.30593. eCollection 2016 Aug. Jundishapur J Microbiol. 2016. PMID: 27800129 Free PMC article.
-
Transfer of plasmid DNA to clinical coagulase-negative staphylococcal pathogens by using a unique bacteriophage.Appl Environ Microbiol. 2015 Apr;81(7):2481-8. doi: 10.1128/AEM.04190-14. Epub 2015 Jan 23. Appl Environ Microbiol. 2015. PMID: 25616805 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
Research Materials
Miscellaneous