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. 2007 Mar 31;121(1-2):131-7.
doi: 10.1016/j.vetmic.2006.11.026. Epub 2006 Dec 2.

Absence of surface expression of feline infectious peritonitis virus (FIPV) antigens on infected cells isolated from cats with FIP

E Cornelissen  1 , H L Dewerchin, E Van Hamme, H J Nauwynck
Affiliations

Absence of surface expression of feline infectious peritonitis virus (FIPV) antigens on infected cells isolated from cats with FIP

E Cornelissen et al. Vet Microbiol. .

Abstract

Feline infectious peritonitis virus (FIPV) positive cells are present in pyogranulomas and exudates from cats with FIP. These cells belong mainly to the monocyte/macrophage lineage. How these cells survive in immune cats is not known. In this study, FIPV positive cells were isolated from pyogranulomas and exudates of 12 naturally FIPV-infected cats and the presence of two immunologic targets, viral antigens and MHC I, on their surface was determined. The majority of the infected cells were confirmed to be cells from the monocyte/macrophage lineage. No surface expression of viral antigens was detected on FIPV positive cells. MHC I molecules were present on all the FIPV positive cells. After cultivation of the isolated infected cells, 52+/-10% of the infected cells re-expressed viral antigens on the plasma membrane. In conclusion, it can be stated that in FIP cats, FIPV replicates in cells of the monocyte/macrophage lineage without carrying viral antigens in their plasma membrane, which could allow them to escape from antibody-dependent cell lysis.

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Figures

Fig. 1
Fig. 1
Surface expression of the monocyte marker (A); absence of surface expression of viral proteins (B); surface expression of MHC I (C) on cytoplasmic FIPV positive cells.
Fig. 2
Fig. 2
Re-expression of viral proteins on the plasma membrane after cultivation of FIPV positive cells isolated from three cats with naturally occurring FIP: immunofluorescence pictures (A); kinetics (B).
See this image and copyright information in PMC

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References

    1. Cammarata Parodi M., Cammarata G., Paltrinieri S., Lavazza A., Ape F. Using direct immunofluorescence to detect coronaviruses in peritoneal and pleural effusions. J. Small Anim. Pract. 1993;34:609–613.
    1. de Groot-Mijnes J.D., van Dun J.M., van der Most R.G., de Groot R.J. Natural history of a recurrent feline coronavirus infection and the role of cellular immunity in survival and disease. J. Virol. 2005;79:1036–1044. - PMC - PubMed
    1. Dewerchin H.L., Cornelissen E., Nauwynck H.J. Replication of feline coronaviruses in peripheral blood monocytes. Arch. Virol. 2005;150:2483–2500. - PMC - PubMed
    1. Dewerchin H.L., Cornelissen E., Nauwynck H.J. Feline infectious peritonitis virus infected monocytes internalize viral membrane-bound proteins upon antibody addition. J. Gen. Virol. 2006;87:1685–1690. - PubMed
    1. Favoreel, H.W., 1999. Antibody-induced clearance of viral and cellular plasma membrane proteins from pseudorabies virus-infected cells, and its possible role in immune evasion. PhD Thesis, University of Ghent.

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