New photoproducts from irradiation of NADH with near-UV light

Abstract

Adenosine 5'-diphosphoribose (ADPR) and a second compound, which may be nicotinamide, are the newly discovered photoproducts resulting from irradiation of beta-nicotinamide adenine dinucleotide (beta-NADH) in the wavelength range of 300-400 nm under oxygen-poor conditions. Both products emerge there even exclusively, whereas, at higher oxygen concentrations, the oxidized form of nicotinamide adenine dinucleotide (NAD+) is additionally formed, although still as a minor product. The development of ADPR and NAD+ is clearly oxygen-dependent, while, for the formation of the second photoproduct, small quantities of oxygen appear to be sufficient.