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. 1991 Oct;229(3):460-6.
doi: 10.1007/BF00267470.

Characterisation of saporin genes: in vitro expression and ribosome inactivation

A P Fordham-Skelton  1 , P N Taylor, M R Hartley, R R Croy
Affiliations

Characterisation of saporin genes: in vitro expression and ribosome inactivation

A P Fordham-Skelton et al. Mol Gen Genet. 1991 Oct.

Abstract

A. Saponaria (soapwort) genomic library was screened with a PCR-derived saporin-specific gene probe. The nucleotide sequences of three saporin genomic clones were determined. One of the clones contained a full-length saporin coding sequence whilst the other two were truncated. A hybrid full-length saporin coding sequence was constructed using the two truncated clones. An SP6 promoter sequence and in-frame initiation codon was added to each of the coding sequences using PCR. In vitro translation of saporin coding sequence transcripts in rabbit reticulocyte lysates resulted in the specific depurination of 28S RNA. This indicated that the saporin sequences encoded functional polypeptides with RNA N-glycosidase activity.

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References

    1. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 - PubMed
    1. J Mol Biol. 1983 Nov 15;170(4):827-42 - PubMed
    1. Arch Biochem Biophys. 1975 Aug;169(2):522-8 - PubMed
    1. Eur J Biochem. 1988 Jan 15;171(1-2):45-50 - PubMed
    1. FEBS Lett. 1986 Jan 20;195(1-2):1-8 - PubMed

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