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. 1991 Nov 15;88(22):10134-7.
doi: 10.1073/pnas.88.22.10134.

A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals

D R Burton  1 , C F Barbas 3rd, M A Persson, S Koenig, R M Chanock, R A Lerner
Affiliations

A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals

D R Burton et al. Proc Natl Acad Sci U S A. .

Abstract

A panel of human monoclonal antibody Fab fragments has been generated against the surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen selection from a random combinatorial library expressed on the surface of filamentous phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly with affinity constants of greater than 10(8) M-1) and notable sequence diversity. Given appropriate donor selection, the methods described should allow the generation of antibodies for the evaluation of passive immunization as a therapy for AIDS.

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References

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    1. Trends Biotechnol. 1991 May;9(5):169-75 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4363-6 - PubMed
    1. Nature. 1991 Aug 15;352(6336):624-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82 - PubMed

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