doi: 10.1038/nmeth988.
Epub 2006 Dec 31.
Optical induction of synaptic plasticity using a light-sensitive channel
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- PMID: 17195846
- DOI: 10.1038/nmeth988
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Optical induction of synaptic plasticity using a light-sensitive channel
Nat Methods.
2007 Feb.
Abstract
We have combined millisecond activation of channelrhodopsin-2 (ChR2), a light-gated ion channel, with two-photon calcium imaging to investigate active synaptic contacts in rat hippocampal slice cultures. Calcium influx was larger during light-induced action potentials than during action potentials induced by somatic current injection, leading to highly reproducible synaptic transmission. Pairing of light stimulation with postsynaptic depolarization induced long-term potentiation, making this technique ideal for genetic and pharmacological dissection of synaptic plasticity.
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