Cholinergic modulation of sensory interference in rat primary somatosensory cortical neurons

Abstract

Sensory interaction was studied using extracellular recordings from 275 neurons in the primary somatosensory (SI) cortex of pentobarbital-anesthetized rats. Tactile stimulation was applied to the receptive field using a 1 mm diameter probe that indented the skin for 20 ms, at 0.5 Hz, (test stimulus). Tactile test responses of SI neurons decreased during simultaneous application of a gentle tickling (distracter stimuli) continuously for 60 s on a separate receptive field located in the same or the contralateral hindlimb (ipsi- or contralateral distraction). This decrease in neural response produced by distracter stimuli was interpreted as "sensory interference". Sensory interference was observed in 66% and 61% of recorded SI neurons when ipsi- or contralateral distracters were applied, respectively and was blocked by a novel stimulus obtained by increasing the stimulation frequency of the test tactile stimuli from 0.5 to 2 Hz. The number of neurons showing sensory interference in response to a contralateral distracter was not modified after corpus callosum transection, suggesting that interhemispheric connections are not crucial for sensory interference. In contrast, the number of neurons showing sensory interference decreased in animals with 192 IgG-saporin basal forebrain lesions that decreased the number of cortical cholinergic fibers. This finding indicates that cholinergic afferents from the basal forebrain are fundamental to sensory interference and suggests that the associative cortices - basal forebrain - sensory cortices network may be implicated in sensory interference.