Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Oct;30(4):650-6.
doi: 10.1128/am.30.4.650-656.1975.

Enzymatic transformation of morphine by hydroxysteroid dehydrogenase from Pseudomonas testosteroni

P Liras, S S Kasparian, W W Umbreit

Enzymatic transformation of morphine by hydroxysteroid dehydrogenase from Pseudomonas testosteroni

P Liras et al. Appl Microbiol. 1975 Oct.

Abstract

Eznyme preparations from Pseudomonas testosteroni containing alpha- and beta- hydroxysteroid dehydrogenases catalyzed the oxidation of morphine and codeine by nicotinamide adenine dinucleotide. Morphine was converted in relatively low yield into 14-hydroxymorphinone probably via morphinone as an intermediate. Codeine was converted to codeinone and 14-hydroxycodeinone. Only the conversions at the 6-position were carred out by the hydroxysteroid dehydrogenase. Hydroxylation at the 14-position did occur spontaneously (or enzymatically with a contaminating enzyme) ater oxidation at the 6-position.

PubMed Disclaimer

References

    1. J Chromatogr. 1975 Mar 19;106(1):238-40 - PubMed
    1. J Biol Chem. 1953 Dec;205(2):823-37 - PubMed
    1. Nature. 1954 Jun 19;173(4416):1189-90 - PubMed
    1. J Biol Chem. 1956 Feb;218(2):661-74 - PubMed
    1. J Biol Chem. 1956 Feb;218(2):675-91 - PubMed

LinkOut - more resources