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. 2006 Dec 28;12(48):7815-20.
doi: 10.3748/wjg.v12.i48.7815.

H pylori infection and systemic antibodies to CagA and heat shock protein 60 in patients with coronary heart disease

Cristina Lenzi  1 , Alberto Palazzuoli, Nicola Giordano, Giuliano Alegente, Catia Gonnelli, Maria-Stella Campagna, Annalisa Santucci, Michele Sozzi, Panagiotis Papakostas, Fabio Rollo, Ranuccio Nuti, Natale Figura
Affiliations

H pylori infection and systemic antibodies to CagA and heat shock protein 60 in patients with coronary heart disease

Cristina Lenzi et al. World J Gastroenterol. .

Abstract

Aim: to determine the overall prevalence of H pylori and CagA positive H pylori infection and the prevalence of other bacterial and viral causes of chronic infection in patients with coronary heart disease (CHD), and the potential role of anti-heat-shock protein 60 (Hsp60) antibody response to these proteins in increasing the risk of CHD development.

Methods: Eighty patients with CHD and 160 controls were employed. We also compared the levels of anti-heat-shock protein 60 (Hsp60) antibodies in the two groups. The H pylori infection and the CagA status were determined serologically, using commercially available enzyme-linked immunosorbent assays (ELISA), and a Western blotting method developed in our laboratory. Systemic antibodies to Hsp60 were determined by a sandwich ELISA, using a polyclonal antibody to Hsp60 to sensitise polystyrene plates and a commercially available human Hsp60 as an antigen.

Results: The overall prevalence of H pylori infection was 78.7% (n = 63) in patients and 76.2% (n = 122) in controls (P = 0.07). Patients infected by CagA-positive (CagA+) H pylori strains were 71.4% (n = 45) vs 52.4% of infected controls (P = 0.030, OR = 2.27). Systemic levels of IgG to Hsp60 were increased in H pylori-negative patients compared with uninfected controls (P < 0.001) and CagA-positive infected patients compared with CagA-positive infected controls (P = 0.007).

Conclusion: CagA positive H pylori infection may concur to the development of CHD; high levels of anti-Hsp60 antibodies may constitute a marker and/or a concomitant pathogenic factor of the disease.

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Figures

Figure 1
Figure 1
Prevalence of overall H pylori and CagA+ H pylori infection in patients and controls.
Figure 2
Figure 2
Mean levels (in optical density) of anti-Hsp60 systemic antibodies in patients and controls.
See this image and copyright information in PMC

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