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. 1991 Nov 15;280 ( Pt 1)(Pt 1):187-91.
doi: 10.1042/bj2800187.

Isolation and overexpression in Escherichia coli of the flavodoxin gene from Anabaena PCC 7119

M F Fillat  1 , W E Borrias, P J Weisbeek
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Isolation and overexpression in Escherichia coli of the flavodoxin gene from Anabaena PCC 7119

M F Fillat et al. Biochem J. .

Abstract

The gene coding for flavodoxin from Anabaena PCC 7119 was cloned by using the polymerase chain reaction (PCR). The gene is transcribed into a 1250-base transcript. The expression of the flavodoxin gene was analysed and found to be regulated at the transcriptional level by the availability of iron. The PCR-amplified gene was cloned into the expression vector pTrc 99b and expressed in Escherichia coli. High concentrations of flavodoxin were found (20% of total protein). The recombinant protein was purified from the cytosolic fraction of the cells and it exhibited properties identical with those of the wild-type Anabaena flavodoxin.

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References

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