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. 2007 Feb;4(2):123-5.
doi: 10.1038/nmeth995. Epub 2007 Jan 7.

Detection of minor drug-resistant populations by parallel allele-specific sequencing

Fangping Cai  1 , Haifeng Chen, Charles B Hicks, John A Bartlett, Jun Zhu, Feng Gao
Affiliations

Detection of minor drug-resistant populations by parallel allele-specific sequencing

Fangping Cai et al. Nat Methods. 2007 Feb.

Erratum in

  • Nat Methods. 2007 Feb;4(2):187

Abstract

We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at approximately 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.

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