Prevalence of Coxiella burnetii DNA in vaginal and uterine samples from healthy cats of north-central Colorado

Abstract

Q fever is a worldwide zoonotic disease caused by Coxiella burnetii. Although traditionally associated with livestock exposure, human infection has also been documented from contact with parturient cats. The goal of this study was to determine the prevalence of C burnetii DNA in uterine and vaginal tissues from healthy client-owned and shelter cats of north-central Colorado using polymerase chain reaction assay. Coxiella burnetii was not amplified from vaginal samples of any cat or uterine biopsies of shelter cats. However, a nucleotide sequence with 99% homology to C burnetii DNA was amplified from four of 47 (8.5%) uterine biopsies of client-owned cats. This study demonstrates that clinically normal cats in north-central Colorado can harbor C burnetii. Care should be taken when attending to parturient cats and contact with parturient secretions should be avoided. Additional studies are indicated to further characterize the role of cats in zoonotic Q fever.

Fig 1

Gel electrophoresis demonstrating Coxiella burnetii PCR. Lane 1: 100 bp DNA ladder. Lane 2: positive feline uterine sample with 687 bp amplicon. Lanes 3–5: negative feline uterine samples. Lane 6: negative control (sterile water template). Lane 7: positive control (C burnetii DNA). Lane 8: 100 bp DNA ladder.