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. 2007 Jan 16;23(2):405-7.
doi: 10.1021/la062950p.

Cholesterol-phospholipid complexation in fluid bilayers as evidenced by nearest-neighbor recognition measurements

Jianbing Zhang  1 , Honghua Cao, Steven L Regen
Affiliations

Cholesterol-phospholipid complexation in fluid bilayers as evidenced by nearest-neighbor recognition measurements

Jianbing Zhang et al. Langmuir. .

Abstract

Nearest-neighbor recognition experiments have been carried out using varying ratios of exchangeable dimer analogs of 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol and 1,2-distearoyl-sn-glycero-3-phosphatidylglycerol in cholesterol-rich unilamellar vesicles at 60 degrees C. Equilibrium dimer distributions that were obtained support a structural model of the liquid-ordered bilayer in which free cholesterol and the longer-chain phospholipid homodimer are in equilibrium with a complex of unique stoichiometry, where one cholesterol molecule combines with two of the long-chain phospholipid homodimers. In this model, the mixing of the short-chain phospholipids with the uncomplexed long-chain phospholipids is ideal, and the complexed dimers are shielded from the disulfide exchange reaction.

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Figures

Figure 1
Figure 1
A plot of Le versus Lf, data taken from Table 1. The solid line was calculated using eq 8 with the parameters K2 = 2021 mol%2 and n = 2.04.
Chart 1
Chart 1
See this image and copyright information in PMC

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