The ligand recognized by ELAM-1 on HL60 cells is not carried by N-linked oligosaccharides

Abstract

The sialyl Lewis-x determinant is a ligand for ELAM-1, a major adhesion molecule for HL60 cells and neutrophils. ELAM-1 expression can selectively be induced on human umbilical vein endothelial cells (HUVEC) by tumor necrosis factor, interleukin 1 and lipopolysaccharide. The determinant sialyl Lewis-x is found on both glycolipids as well as on glycoproteins. Using specific inhibitors of the biosynthesis of N-linked glycosylated glycoproteins, we investigated whether N-linked glycans or their modifications are involved in ELAM-1-dependent adhesion of HL60 cells to activated HUVEC. The inhibitors of glycoprotein processing N-methyl-deoxynojirimycin, 1-deoxymannojirimycin and swainsonine did not affect ELAM-1-dependent adhesion. Complex-type N-linked glycans are not required for ELAM-1 mediated adhesion, and therefore the ligand for ELAM-1 is most likely a glycolipid, or a glycoprotein carrying O-linked oligosaccharides.