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. 2007;46(8):1318-20.
doi: 10.1002/anie.200603817.

Patterned paper as a platform for inexpensive, low-volume, portable bioassays

Andres W Martinez  1 , Scott T Phillips, Manish J Butte, George M Whitesides
Affiliations

Patterned paper as a platform for inexpensive, low-volume, portable bioassays

Andres W Martinez et al. Angew Chem Int Ed Engl. 2007.
No abstract available

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Figures

Figure 1
Figure 1
Chromatography paper patterned with photoresist. The darker lines are cured photoresist; the lighter areas are unexposed paper. (a) Patterned paper after absorbing 5 μL of Waterman red ink by capillary action. The central channel absorbs the sample by capillary action and the pattern directs the sample into three separate test areas. (b) Complete assay after spotting the reagents. The square region on the right is the protein test and the circular region on the left is the glucose test. The circular region on the top was used as a control well and was spotted with either iodide but no enzyme solution, or with enzyme solution but no iodide. (c) Negative control for glucose (left) and protein (right) using 5 μL of an artificial urine solution.[18] (d) Positive assay for glucose (left) and protein (right) using 5 μL of a solution that contained 550 mM glucose and 75 μM BSA in an artificial urine solution. The control well was spotted with the potassium iodide solution, but not with the enzyme solution. A similar control containing the enzyme solution, but not the iodide, gave identical results (data not shown). (e) Glucose and protein detection assays using varying concentrations of glucose and BSA.
Figure 2
Figure 2
Assays contaminated with (a) dirt, (b) plant pollen, and (c) graphite powder. The pictures were taken before and after running an artificial urine solution that contained 550 mM glucose and 75 μM BSA. The particulates do not move up the channels during the assay.
Scheme 1
Scheme 1
Schematic diagram depicting the method for patterning paper into millimeter-sized channels: (a) Photolithography was used to pattern SU-8 photoresist embedded into paper; (b) The patterned paper was modified for bioassays.
See this image and copyright information in PMC

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