Review
doi: 10.1139/o06-189.
Investigating membrane protein dynamics in living cells
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- PMID: 17215870
- DOI: 10.1139/o06-189
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Review
Investigating membrane protein dynamics in living cells
Biochem Cell Biol.
2006 Dec.
Abstract
Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.
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