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Comparative Study
. 1991 Dec 15;88(24):11393-7.
doi: 10.1073/pnas.88.24.11393.

Prohormone processing in Xenopus oocytes: characterization of cleavage signals and cleavage enzymes

J Korner  1 , J Chun, L O'Bryan, R Axel
Affiliations
Comparative Study

Prohormone processing in Xenopus oocytes: characterization of cleavage signals and cleavage enzymes

J Korner et al. Proc Natl Acad Sci U S A. .

Erratum in

  • Proc Natl Acad Sci U S A 1992 May 15;89(10):4779

Abstract

In this study, we characterize the sequences required for the cleavage of prohormones in Xenopus oocytes. We demonstrate that the yeast alpha-factor and the Aplysia egg-laying hormone (ELH) precursors are not cleaved in oocytes following simple pairs of basic residues, such as Lys-Arg, but that the ELH precursor is cleaved following the consensus sequence Arg-Xaa-(Lys/Arg)-Arg. This motif is conserved among precursors that are cleaved in virtually all mammalian cell types. Mutations that generate this sequence in the alpha-factor prohormone also result in efficient processing within oocytes. Cleavage at this consensus sequence may be due to the action of the Xenopus homologues of mammalian furin.

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References

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