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. 2007 Mar;14(3):331-3.
doi: 10.1128/CVI.00351-06. Epub 2007 Jan 17.

Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Lia M Haynes  1 , Congrong Miao, Jennifer L Harcourt, Joel M Montgomery, Mai Quynh Le, Sergey A Dryga, Kurt I Kamrud, Bryan Rivers, Gregory J Babcock, Jennifer Betts Oliver, James A Comer, Mary Reynolds, Timothy M Uyeki, Daniel Bausch, Thomas Ksiazek, William Thomas, Harold Alterson, Jonathan Smith, Donna M Ambrosino, Larry J Anderson
Affiliations

Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Lia M Haynes et al. Clin Vaccine Immunol. 2007 Mar.

Abstract

Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

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Figures

FIG. 1.
FIG. 1.
Scatter chart of absorbance values with representative sera from both SARS patients and healthy controls for IgG antibodies to recombinant spike protein (A) and recombinant nucleocapsid protein (B). Specimens were obtained from 61 SARS patients at 2 to 150 days postonset of symptoms and from 384 healthy U.S. blood donors. Results are plotted as A405 values with a 490-nm reference filter. The black line indicates the cutoff values of 0.201 (A) and 0.210 (B) for each assay.
See this image and copyright information in PMC

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