Application of quantum dots to multicolor flow cytometry
- PMID: 17237539
- DOI: 10.1385/1-59745-369-2:175
Application of quantum dots to multicolor flow cytometry
Abstract
The coupling of quantum dots (QDs) to antibodies heralds a new era of versatility in multicolor flow cytometry. This chapter introduces the properties of QDs (as relevant to flow cytometric applications), the advantages derived from these properties, and the procedure for conjugating antibodies to QDs. Finally, we discuss strategies for choosing the combinations of QDs and antibodies best suited for multicolor experiments.
Similar articles
-
Quantum dots in flow cytometry.Methods Mol Biol. 2007;374:185-203. doi: 10.1385/1-59745-369-2:185. Methods Mol Biol. 2007. PMID: 17237540
-
The use of quantum dot nanocrystals in multicolor flow cytometry.Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Jul-Aug;2(4):334-48. doi: 10.1002/wnan.75. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010. PMID: 20101649 Review.
-
Flow cytometric analysis to detect pathogens in bacterial cell mixtures using semiconductor quantum dots.Anal Chem. 2008 Feb 1;80(3):864-72. doi: 10.1021/ac7018365. Epub 2008 Jan 11. Anal Chem. 2008. PMID: 18186615
-
Quantum Dots as alternatives to organic fluorophores for Cryptosporidium detection using conventional flow cytometry and specific monoclonal antibodies: lessons learned.Cytometry A. 2007 Apr;71(4):265-71. doi: 10.1002/cyto.a.20381. Cytometry A. 2007. PMID: 17279568
-
Quantum dot technology in flow cytometry.Methods Cell Biol. 2011;102:463-77. doi: 10.1016/B978-0-12-374912-3.00018-3. Methods Cell Biol. 2011. PMID: 21704850 Review.
Cited by
-
Laser Sources for Traditional and Spectral Flow Cytometry.Methods Mol Biol. 2024;2779:33-68. doi: 10.1007/978-1-0716-3738-8_3. Methods Mol Biol. 2024. PMID: 38526781 Review.
-
CD8+ cellular immunity mediates rAd5 vaccine protection against Ebola virus infection of nonhuman primates.Nat Med. 2011 Aug 21;17(9):1128-31. doi: 10.1038/nm.2447. Nat Med. 2011. PMID: 21857654
-
The role of colorectal cancer stem cells in metastatic disease and therapeutic response.Cancers (Basel). 2011 Jan 1;3(1):319-39. doi: 10.3390/cancers3010319. Cancers (Basel). 2011. PMID: 21318087 Free PMC article.
-
Optimization and qualification of an 8-color intracellular cytokine staining assay for quantifying T cell responses in rhesus macaques for pre-clinical vaccine studies.J Immunol Methods. 2012 Dec 14;386(1-2):10-21. doi: 10.1016/j.jim.2012.08.011. Epub 2012 Aug 28. J Immunol Methods. 2012. PMID: 22955212 Free PMC article.
-
Comparison of plasma viremia and antibody responses in macaques inoculated with envelope variants of single-cycle simian immunodeficiency virus differing in infectivity and cellular tropism.J Virol. 2008 Jan;82(1):321-34. doi: 10.1128/JVI.01094-07. Epub 2007 Oct 17. J Virol. 2008. PMID: 17942538 Free PMC article.
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources