Non-Mendelian Female Sterility in DROSOPHILA MELANOGASTER: Characterization of the Noninducer Chromosomes of Inducer Strains

Abstract

In relation to non-Mendelian female sterility, Drosophila melanogaster strains can be divided into two main classes, inducer and reactive. The genetic element responsible for the inducer condition (I factor) is chromosomal and may be linked to any inducer-strain chromosome. Each chromosome carrying the I factor (i(+) chromosome) can, when introduced by the paternal gamete into a reactive oocyte, give rise to females (denoted SF) showing more-or-less reduced fertility. As long as i(+) chromosomes are transmitted through heterozygous males with reactive originating chromosomes (r chromosomes), I factor follows Mendelian segregation patterns. In contrast, in heterozygous i(+)/r females, a varying proportion of r chromosomes may irreversibly acquire I factor, independently of classical genetic recombination, by a process called chromosomal contamination. The contaminated reactive chromosomes behave as i(+) chromosomes.-In the present paper, evidence is given that the Luminy inducer strain displays a polymorphism for two kinds of second chromosomes. Some of them are i(+), while others, denoted i(o), are unable to induce any SF sterility when introduced by paternal gametes into reactive oocytes. They are also unable to induce contamination of r chromosomes, but, like r chromosomes, they may be contaminated by i(+) chromosomes in SF or RSF females. The study of the segregation of i(+) and i(o) second chromosomes in the progeny of heterozygous Luminy males and females leads to the conclusion that on chromosome 2 of the Luminy stock the I factor is at a single locus. -X, second and third i(o) chromosomes have been found in several inducer strains. Since these chromosomes can be maintained with i(+) chromosomes in inducer strains in spite of their ability to be contaminated in RSF females, it can be concluded that chromosomal contamination does not take place in females of inducer strains. This implies that contamination occurs only in cells having cytoplasm in a reactive state.