Maximum likelihood estimation of linkage and interference from tetrad data

Abstract

Maximum likelihood equations have been derived for estimation of map distance and interference from two-point and ranked tetrad data. The estimators have been applied to data from Saccharomyces cerevisiae and Schizosaccharomyces pombe. S. cerevisiae consistently shows quite strong interference over the mapped genome. In striking contrast, S. pombe consistently shows much weaker interference and many crosses exhibit negative interference. In neither species was there a conspicuous tendency for intervals spanning a centromere to show less interference than those that did not. Since the amount of recombination per microgram of DNA in the two species is similar, the difference in interference characteristics seems to be a reflection of some fundamental difference in the recombination process of the two species.