Identification of phosphotyrosine residues during protein sequence analysis
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- PMID: 1724944
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Identification of phosphotyrosine residues during protein sequence analysis
Pept Res.
1991 Jan-Feb.
Abstract
Synthetic tyrosine-phosphorylated peptides were subjected to protein sequence analysis using a gas-phase sequencer and on-line phenylthiohydantoin (PTH) amino acid analysis. Our data show that phosphotyrosine is stable to the gas-phase sequencing chemistry and can be detected as its PTH-derivative during routine sequence analysis without the need of prior tyrosine radiolabeling.
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