High rate of detection of mixed infections of Plasmodium vivax and Plasmodium falciparum in South-East of Iran, using nested PCR
- PMID: 17257891
- DOI: 10.1016/j.parint.2006.12.001
High rate of detection of mixed infections of Plasmodium vivax and Plasmodium falciparum in South-East of Iran, using nested PCR
Abstract
Sistan and Baluchestan province, South-East of Iran, has been reported as an endemic area of malaria [Sadrizadeh B. Malaria in the world, in the eastern Mediterranean region and in Iran: Review article. WHO/EMRO Report 2001: 1-13.]. The main objective of this research was to perform rapid and correct diagnoses of malaria infection. Blood specimens were collected from 140 suspected volunteers. The Giemsa-stained slides examination and nested PCR for amplification of the Plasmodium small subunit ribosomal genes (ssrRNA) were utilized. The results demonstrated 118 out of 140 cases (84.3%) positive for malaria parasites, including 60.7%, 20.7% and 2.9% as having Plasmodium vivax (P.v), Plasmodium falciparum (P.f) and mixed infections (P.v+P.f), respectively by microscopy. The nested PCR detected malaria parasites in 134 samples (94.3%), consisting of 51.4% P.v, 12.6% P.f and 29.3% mixed infections. The PCR analysis detected 37 cases of mixed infections more than that of the routine microscopy. These results suggested that there are a considerable number of cases with mixed infections in the study area that mainly remain undiagnosed by microscopy. It is also concluded that the nested PCR is a suitable complement to microscopy for accurate specific diagnosis of malaria species in field.
Similar articles
-
Molecular evidence of mixed P. vivax and P. falciparum infections in northern Islamic Republic of Iran.East Mediterr Health J. 2004 May;10(3):336-42. East Mediterr Health J. 2004. PMID: 16212210
-
Comparison of microscopical examination and semi-nested multiplex polymerase chain reaction in diagnosis of Plasmodium falciparum and P. vivax.East Mediterr Health J. 2011 Jan;17(1):51-5. East Mediterr Health J. 2011. PMID: 21735802
-
Polymerase chain reaction detection of Plasmodium vivax and Plasmodium falciparum DNA from stored serum samples: implications for retrospective diagnosis of malaria.Am J Trop Med Hyg. 2007 Sep;77(3):444-6. Am J Trop Med Hyg. 2007. PMID: 17827357
-
Markers for population genetic analysis of human plasmodia species, P. falciparum and P. vivax.J Vector Borne Dis. 2003 Sep-Dec;40(3-4):78-83. J Vector Borne Dis. 2003. PMID: 15119076 Review.
-
Genetic diversity and population history of Plasmodium falciparum and Plasmodium vivax.Parassitologia. 2006 Dec;48(4):561-6. Parassitologia. 2006. PMID: 17688177 Review. No abstract available.
Cited by
-
Efficiency of Nested-PCR in Detecting Asymptomatic Cases toward Malaria Elimination Program in an Endemic Area of Iran.Iran J Parasitol. 2015 Jan-Mar;10(1):39-45. Iran J Parasitol. 2015. PMID: 25904944 Free PMC article.
-
On the evolution of reproductive restraint in malaria.Proc Biol Sci. 2008 May 22;275(1639):1217-24. doi: 10.1098/rspb.2007.1545. Proc Biol Sci. 2008. PMID: 18303001 Free PMC article.
-
Plasmodium vivax MSP-3β Gene as a Genetic Marker for the Parasite Detection in Comparison with Ssrrna Gene.Iran J Public Health. 2010;39(2):105-9. Epub 2010 Jun 30. Iran J Public Health. 2010. PMID: 23113014 Free PMC article.
-
Summary of discordant results between rapid diagnosis tests, microscopy, and polymerase chain reaction for detecting Plasmodium mixed infection: a systematic review and meta-analysis.Sci Rep. 2020 Jul 29;10(1):12765. doi: 10.1038/s41598-020-69647-y. Sci Rep. 2020. PMID: 32728145 Free PMC article.
-
Detection of mixed-species infections of Plasmodium falciparum and Plasmodium vivax by nested PCR and rapid diagnostic tests in southeastern Iran.Am J Trop Med Hyg. 2015 Jul;93(1):181-5. doi: 10.4269/ajtmh.14-0650. Epub 2015 May 11. Am J Trop Med Hyg. 2015. PMID: 25962771 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources