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. 2007 Jun;87(6):1477-9.
doi: 10.1016/j.fertnstert.2006.11.059. Epub 2007 Jan 25.

Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques

Jeffrey T Jensen  1 , Jessica E Stanley, Mary B Zelinski, Richard L Stouffer, Darla Jacob, John Fanton
Affiliations

Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques

Jeffrey T Jensen et al. Fertil Steril. 2007 Jun.

Abstract

To determine the practicality of controlled ovulation of the dominant follicle as a technique to study meiotic maturation of oocytes during contraceptive research, we developed a technique for aspiration of the single dominant follicle using a dual-needle continuous irrigation technique 27 hours after an ovulatory stimulus. All of the oocytes (3/3) recovered from control animals, but only 1/6 (17%) of oocytes from animals treated with the meiotic inhibitor ORG 9935 exhibited germinal vesicle breakdown, indicating resumption of meiosis.

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Figures

FIGURE 1
FIGURE 1
(A) The 22-gage aspiration and irrigation needles are inserted percutaneously, and positioned for simultaneous entry into the follicle. The follicle is then aspirated (B) and irrigated (C) continuously to ensure recovery of the oocyte.
See this image and copyright information in PMC

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