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. 2007 Jan 29:7:2.
doi: 10.1186/1471-2385-7-2.

Comparison of manual and semi-automated delineation of regions of interest for radioligand PET imaging analysis

Tiffany W Chow  1 , Shinichiro Takeshita, Kie Honjo, Christina E Pataky, Peggy L St Jacques, Maggie L Kusano, Curtis B Caldwell, Joel Ramirez, Sandra Black, Nicolaas P L G Verhoeff
Affiliations

Comparison of manual and semi-automated delineation of regions of interest for radioligand PET imaging analysis

Tiffany W Chow et al. BMC Nucl Med. .

Abstract

Background: As imaging centers produce higher resolution research scans, the number of man-hours required to process regional data has become a major concern. Comparison of automated vs. manual methodology has not been reported for functional imaging. We explored validation of using automation to delineate regions of interest on positron emission tomography (PET) scans. The purpose of this study was to ascertain improvements in image processing time and reproducibility of a semi-automated brain region extraction (SABRE) method over manual delineation of regions of interest (ROIs).

Methods: We compared 2 sets of partial volume corrected serotonin 1a receptor binding potentials (BPs) resulting from manual vs. semi-automated methods. BPs were obtained from subjects meeting consensus criteria for frontotemporal degeneration and from age- and gender-matched healthy controls. Two trained raters provided each set of data to conduct comparisons of inter-rater mean image processing time, rank order of BPs for 9 PET scans, intra- and inter-rater intraclass correlation coefficients (ICC), repeatability coefficients (RC), percentages of the average parameter value (RM%), and effect sizes of either method.

Results: SABRE saved approximately 3 hours of processing time per PET subject over manual delineation (p < .001). Quality of the SABRE BP results was preserved relative to the rank order of subjects by manual methods. Intra- and inter-rater ICC were high (>0.8) for both methods. RC and RM% were lower for the manual method across all ROIs, indicating less intra-rater variance across PET subjects' BPs.

Conclusion: SABRE demonstrated significant time savings and no significant difference in reproducibility over manual methods, justifying the use of SABRE in serotonin 1a receptor radioligand PET imaging analysis. This implies that semi-automated ROI delineation is a valid methodology for future PET imaging analysis.

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Figure 1
Figure 1
Region of interest delineation: a) manual, b) using SABRE.
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References

    1. Dade LA, Gao FQ, Kovacevic N, Roy P, Rockel C, O'Toole CM, Lobaugh NJ, Feinstein A, Levine B, Black SE. Semiautomatic brain region extraction: A method of parcellating brain regions from structural magnetic resonance images. Neuroimage. 2004;22:1492–1502. doi: 10.1016/j.neuroimage.2004.03.023. - DOI - PubMed
    1. Kim JJ, Crespo-Facorro B, Andreasen NC, O'Leary DS, Zhang B, Harris G, Magnotta VA. An MRI-based parcellation method for the temporal lobe. Neuroimage. 2000;11:271–288. doi: 10.1006/nimg.2000.0543. - DOI - PubMed
    1. Caviness Jr VS, Meyer J, Makris N, Kennedy DN. MRI-based topographic parcellation of human neocortex: An anatomically specified method with estimate of reliability. Journal of Cognitive Neuroscience. 1996;8:566–587. - PubMed
    1. Fischl B, Salat DH, Busa E, Albert M, Dieterich M, Haselgrove C, van der Kouwe A, Killiany R, Kennedy D, Klaveness S. Whole brain segmentation: Automated labeling of neuroanatomical structures in the human brain. Neuron. 2002;33:341–355. doi: 10.1016/S0896-6273(02)00569-X. - DOI - PubMed
    1. Chan D, Fox NC, Jenkins R, Scahill RI, Crum WR, Rossor MN. Rates of global and regional cerebral atrophy in AD and frontotemporal dementia. Neurology. 2001;27:1756–1763. - PubMed