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. 2007 Apr;51(4):1553-5.
doi: 10.1128/AAC.01405-06. Epub 2007 Jan 29.

First identification of Pseudomonas aeruginosa isolates producing a KPC-type carbapenem-hydrolyzing beta-lactamase

Maria Virginia Villegas  1 , Karen Lolans, Adriana Correa, Juan Nicolas Kattan, Jaime A Lopez, John P Quinn; Colombian Nosocomial Resistance Study Group
Affiliations

First identification of Pseudomonas aeruginosa isolates producing a KPC-type carbapenem-hydrolyzing beta-lactamase

Maria Virginia Villegas et al. Antimicrob Agents Chemother. 2007 Apr.

Abstract

In Medellin, Colombia, three Pseudomonas aeruginosa isolates with high-level carbapenem resistance (MIC>or=256 microg/ml) and an isolate of Citrobacter freundii with reduced susceptibility to imipenem produced the plasmid-mediated class A carbapenemase KPC-2. This is the first report of a KPC-type beta-lactamase identified outside of the family Enterobacteriaceae.

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Figures

FIG. 1.
FIG. 1.
Localization of the blaKPC-2 gene. (A) Electrophoretic profiles of plasmids digested with the indicated enzyme. (B) Hybridization with a blaKPC-2-specific probe. Lanes: 1 to 3, P. aeruginosa PA2404, PA4012, and PA4036, respectively; 4, C. freundii CF6010; 5, KPN633 (positive control) (22); M, digoxygenin-labeled marker; T and S, TEM-10- and SHV-12-containing plasmids, respectively (negative controls).
FIG. 2.
FIG. 2.
Localization of the blaKPC gene in I-CeuI-generated, pulsed-field gel electrophoresis-separated chromosome fragments of P. aeruginosa and C. freundii. (A) I-CeuI fragment restriction pattern. (B) Hybridization with a probe specific to 16S rRNA genes. (C) Hybridization with a probe specific to blaKPC. Lanes: 1, P. aeruginosa ATCC 27853; 2, P. aeruginosa PA4036; 3, C. freundii CH32 (imipenem-sensitive isolate); 4, C. freundii CF6010; M, lambda DNA ladder standard with sizes (in kilobases) indicated on the left.
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