Sporozoites and merozoites of Cryptosporidium parvum share a common epitope recognized by a monoclonal antibody and two-dimensional electrophoresis
- PMID: 1726328
Sporozoites and merozoites of Cryptosporidium parvum share a common epitope recognized by a monoclonal antibody and two-dimensional electrophoresis
Abstract
Sporozoites and merozoites of Cryptosporidium parvum were analyzed for the presence of a 15 kDa surface antigen using a monoclonal antibody probe. Both were found to possess the antigen by immunofluorescence, and further analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed these observations. When separated by two-dimensional electrophoresis the isoelectric point was found to be similar, with major spots at 4.25 and minor spots at 4.15.
Similar articles
-
Protective monoclonal antibody defines a circumsporozoite-like glycoprotein exoantigen of Cryptosporidium parvum sporozoites and merozoites.J Immunol. 1997 Feb 15;158(4):1787-95. J Immunol. 1997. PMID: 9029117
-
Cross-reactivities with Cryptosporidium spp. by chicken monoclonal antibodies that recognize avian Eimeria spp.Vet Parasitol. 2005 Mar 10;128(1-2):47-57. doi: 10.1016/j.vetpar.2004.11.004. Epub 2004 Dec 15. Vet Parasitol. 2005. PMID: 15725532
-
Cryptosporidium parvum merozoites share neutralization-sensitive epitopes with sporozoites.J Immunol. 1990 Jul 1;145(1):298-304. J Immunol. 1990. PMID: 2193057
-
Plasmodium vivax: a monoclonal antibody recognizes a circumsporozoite protein precursor on the sporozoite surface.Exp Parasitol. 1998 Nov;90(3):203-11. doi: 10.1006/expr.1998.4334. Exp Parasitol. 1998. PMID: 9806864
-
[Research advances in the pathogenicity associated proteins of Cryptosporidium parvum].Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2004 Aug;22(4):253-5. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2004. PMID: 15587165 Review. Chinese. No abstract available.
Cited by
-
Tension-type headache.Nat Rev Dis Primers. 2021 Mar 25;7(1):24. doi: 10.1038/s41572-021-00257-2. Nat Rev Dis Primers. 2021. PMID: 33767185 Review.
-
Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15- and 45-kilodalton zoite surface antigen products.Infect Immun. 2000 Jul;68(7):4117-34. doi: 10.1128/IAI.68.7.4117-4134.2000. Infect Immun. 2000. PMID: 10858229 Free PMC article.
-
Cloning and expression of a cDNA encoding epitopes shared by 15- and 60-kilodalton proteins of Cryptosporidium parvum sporozoites.Infect Immun. 1993 Jun;61(6):2377-82. doi: 10.1128/iai.61.6.2377-2382.1993. Infect Immun. 1993. PMID: 7684726 Free PMC article.
-
Cryptosporidium taxonomy: recent advances and implications for public health.Clin Microbiol Rev. 2004 Jan;17(1):72-97. doi: 10.1128/CMR.17.1.72-97.2004. Clin Microbiol Rev. 2004. PMID: 14726456 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources