Anti-phosphotyrosine antibodies--obtaining and purification
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- PMID: 1726504
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Anti-phosphotyrosine antibodies--obtaining and purification
Roum Arch Microbiol Immunol.
1991 Jul-Sep.
Abstract
Anti-phosphotyrosine antibodies were obtained by rabbits immunization using bovine serum albumin-phosphotyrosine complex. Hyperimmune rabbit serum was purified on affinity chromatography column obtained by phosphotyramine binding on Sepharose 4 B, CNBr-activated. The antibodies obtained after purification were tested by ELISA and immunoblotting.
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