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. 1991 Jul-Sep;50(3):187-92.

Anti-phosphotyrosine antibodies--obtaining and purification

M Stefănescu  1 , L Cremer, C Cristescu, C Matache, G Szegli
Affiliations
  • PMID: 1726504

Anti-phosphotyrosine antibodies--obtaining and purification

M Stefănescu et al. Roum Arch Microbiol Immunol. 1991 Jul-Sep.

Abstract

Anti-phosphotyrosine antibodies were obtained by rabbits immunization using bovine serum albumin-phosphotyrosine complex. Hyperimmune rabbit serum was purified on affinity chromatography column obtained by phosphotyramine binding on Sepharose 4 B, CNBr-activated. The antibodies obtained after purification were tested by ELISA and immunoblotting.

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