Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Mar;17(2):193-9.
doi: 10.1007/s10895-007-0158-4. Epub 2007 Jan 31.

Fluorescence assay based on aptamer-quantum dot binding to Bacillus thuringiensis spores

Milada Ikanovic  1 , Walter E Rudzinski, John G Bruno, Amity Allman, Maria P Carrillo, Sulatha Dwarakanath, Suneetha Bhahdigadi, Poornima Rao, Johnathan L Kiel, Carrie J Andrews
Affiliations

Fluorescence assay based on aptamer-quantum dot binding to Bacillus thuringiensis spores

Milada Ikanovic et al. J Fluoresc. 2007 Mar.

Abstract

A novel assay was developed for the detection of Bacillus thuringiensis (BT) spores. The assay is based on the fluorescence observed after binding an aptamer-quantum dot conjugate to BT spores. The in vitro selection and amplification technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment) was used in order to identify the DNA aptamer sequence specific for BT. The 60 base aptamer was then coupled to fluorescent zinc sulfide-capped, cadmium selenide quantum dots (QD). The assay is semi-quantitative, specific and can detect BT at concentrations of about 1,000 colony forming units/ml.

PubMed Disclaimer

References

    1. Biosens Bioelectron. 1999 May 31;14(5):457-64 - PubMed
    1. J Am Chem Soc. 2003 Apr 23;125(16):4771-8 - PubMed
    1. Science. 1998 Sep 25;281(5385):2016-8 - PubMed
    1. Trends Genet. 2001 Apr;17(4):193-9 - PubMed
    1. Proc Natl Acad Sci U S A. 2004 Oct 19;101(42):15027-32 - PubMed

Publication types

MeSH terms

LinkOut - more resources