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Review
. 2007 Jun;82(6):463-72.
doi: 10.1002/ajh.20707.

Cryopreservation of hematopoietic stem cells

David Berz  1 , Elise M McCormack, Eric S Winer, Gerald A Colvin, Peter J Quesenberry
Affiliations
Review

Cryopreservation of hematopoietic stem cells

David Berz et al. Am J Hematol. 2007 Jun.

Abstract

Stem cell transplantation represents a critical approach for the treatment of many malignant and non-malignant diseases. The foundation for these approaches is the ability to cryopreserve marrow cells for future use. This technique is routinely employed in all autologous settings and is critical for cord blood transplantation. A variety of cryopreservatives have been used with multiple freezing and thawing techniques as outlined in the later chapters. Freezing efficiency has been proven repeatedly and the ability of long-term stored marrow to repopulate has been established. Standard approaches outlined here are used in many labs as the field continues to evolve.

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Figures

Fig. 1
Fig. 1
Clonogenic assays. Cells are plated in soft agar and incubated. At set time periods, the cell colonies are counted; this provides an in vitro surrogate of hematopoietic reconstitution potential. (A) CFU/GM (B) CFU/GEMMA.
Fig. 2
Fig. 2
The cryopreservation process for bone marrow, peripheral blood stem cells, and UCB.
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