Cell-free translation system using phosphorothioate-containing mRNA
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- PMID: 1726804
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Cell-free translation system using phosphorothioate-containing mRNA
Nucleic Acids Symp Ser.
1991.
Abstract
Phosphorothioate-containing RNAs were generated by transcription of template DNA using the Sp diastereomers of ribonucleoside 5'-O-(1-thiotriphosphates) (NTP alpha S) and T7 RNA polymerase. The substitution of mRNA by phosphorothioate increased the efficiency of protein synthesis by stabilizing the mRNAs in prokaryotic cell-free translation systems. The substituted mRNAs were also shown to be applicable to the continuous cell-free translation system developed by Spirin and coworkers.
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