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. 1991:(25):151-2.

Cell-free translation system using phosphorothioate-containing mRNA

T Ueda  1 , H Tohda, N Chikazumi, F Eckstein, K Watanabe
Affiliations
  • PMID: 1726804

Cell-free translation system using phosphorothioate-containing mRNA

T Ueda et al. Nucleic Acids Symp Ser. 1991.

Abstract

Phosphorothioate-containing RNAs were generated by transcription of template DNA using the Sp diastereomers of ribonucleoside 5'-O-(1-thiotriphosphates) (NTP alpha S) and T7 RNA polymerase. The substitution of mRNA by phosphorothioate increased the efficiency of protein synthesis by stabilizing the mRNAs in prokaryotic cell-free translation systems. The substituted mRNAs were also shown to be applicable to the continuous cell-free translation system developed by Spirin and coworkers.

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