Tryptic mapping by reversed phase liquid chromatography

Abstract

This chapter reviews the technique of tryptic mapping by reversed phase liquid chromatography by describing its fundamental principles, present status, and recent advances as well as its uses and limitations. Two commonly used S-carboxymethylation/tryptic digestion procedures are documented. Column and instrumental requirements are described and illustrated with examples. Mobile phase selection and operating variables (tG, F) are also discussed in terms of their effects on both peptide resolution and detection sensitivity.