doi: 10.1111/j.1471-4159.1992.tb09288.x.
Purification of neuropathy target esterase from avian brain after prelabelling with [3H]diisopropyl phosphorofluoridate
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- PMID: 1727426
- DOI: 10.1111/j.1471-4159.1992.tb09288.x
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Purification of neuropathy target esterase from avian brain after prelabelling with [3H]diisopropyl phosphorofluoridate
J Neurochem.
1992 Jan.
Abstract
Neuropathy target esterase from hen brains was radiolabelled at the active site with [3H]diisopropyl phosphorofluoridate. The labelled protein was purified by differential centrifugation and Nonidet P40 solubilization, detergent phase partitioning, anion exchange, and preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The volatilizable counts assay and analytical SDS-PAGE were used to monitor the protein. The 150-kDa subunit polypeptide appears as a single band on analytical SDS-PAGE.
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