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. 2007 Apr 1;17(7):1912-5.
doi: 10.1016/j.bmcl.2007.01.040. Epub 2007 Jan 24.

Degradation of DNA by bisulfite treatment

Kazuo Tanaka  1 , Akimitsu Okamoto
Affiliations

Degradation of DNA by bisulfite treatment

Kazuo Tanaka et al. Bioorg Med Chem Lett. .

Abstract

A significant level of target degradation was caused by bisulfite treatment for methylcytosine-selective hydrolysis. The depyrimidination proceeded via addition of bisulfite to pyrimidines in DNA. The quantification with real-time PCR after conventional bisulfite treatment showed a large decrease in the amount of full-length DNA.

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