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. 2007;46(12):1999-2001.
doi: 10.1002/anie.200604546.

Detecting force-induced molecular transitions with fluorescence resonant energy transfer

Peter B Tarsa #  1 , Ricardo R Brau #  1 , Mariya Barch #  2 , Jorge M Ferrer  1 , Yelena Freyzon  3 , Paul Matsudaira  1 , Matthew J Lang  1
Affiliations

Detecting force-induced molecular transitions with fluorescence resonant energy transfer

Peter B Tarsa et al. Angew Chem Int Ed Engl. 2007.
No abstract available

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Figures

Figure 1
Figure 1
Experimental assay design (see Experimental Section for details). DNA hairpin complexes, labeled with opposing Cy3 and Alexa 647 fluorophores, were mechanically loaded by translating the coverslip, as the position of the trapped bead and the emission of the fluorophores were simultaneously monitored. The optical trap and the evanescent fluorescence-excitation field are depicted in the background in red and green, respectively. The inset cartoons show detail of the hairpin conformational change and the expected fluorophore emission (filled circles).
Figure 2
Figure 2
Mechanically induced conformational changes monitored with FRET spectroscopy. A) A DNA hairpin was manipulated with optical tweezers between open or closed conformational states (black) that transition at loads of approximately 18 pN. The state of the hairpin was revealed by FRET between the donor Cy3 (green) and the acceptor Alexa 647 (red). The donor photobleaches at approximately 65 s, confirming that a single FRET pair was monitored. B) Detail of a single hairpin opening transition accompanied by a simultaneous change in FRET, as highlighted by the gray dashed line. The inset cartoons depict the state of the hairpin.
See this image and copyright information in PMC

References

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