Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Oct;72(10):3849-52.
doi: 10.1073/pnas.72.10.3849.

Partial purification of a glucocorticoid receptor

D Failla, G M Tomkins, D V Santi

Partial purification of a glucocorticoid receptor

D Failla et al. Proc Natl Acad Sci U S A. 1975 Oct.

Abstract

A simple method for purification of the glucocorticoid receptor from hepatoma tissue culture cells has been developed. The procedure, which requires only about 24 hr, involves biospecific adsorption of the receptor to deoxycorticosterone derivatized agarose, elution with a glucocorticoid, and gel filtration. The receptor-steroid complex is obtained in 35-40% yield and is about 2000-fold purified. It possesses properties similar to those reported in crude extracts, including sedimentation coefficient in glycerol gradients and activation-dependent binding to nuclei.

PubMed Disclaimer

References

    1. J Lipid Res. 1967 Mar;8(2):142-5 - PubMed
    1. J Mol Biol. 1973 Sep 25;79(3):539-54 - PubMed
    1. Biochemistry. 1973 Jun 19;12(13):2412-6 - PubMed
    1. J Biol Chem. 1973 Aug 25;248(16):5866-72 - PubMed
    1. J Biol Chem. 1973 Sep 25;248(18):6543-58 - PubMed

Publication types

LinkOut - more resources