Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975;49(1):73-80.
doi: 10.1007/BF02175598.

Isolation of herpes simplex virus clones and drug resistant mutants in microcultures

R J Klein

Isolation of herpes simplex virus clones and drug resistant mutants in microcultures

R J Klein. Arch Virol. 1975.

Abstract

Suitable dilutions of herpes simplex virus (HSV) preparations inoculated into microcultures of confluent monolayers of human foreskin or Vero cells, in individual wells of plastic "microplates", induced viral cytopathic effects that resulted from the infection of the cultures by single virus particles. The clonal nature of the viral progeny in isolated wells was supported by visual control over the development of viral foci and by statistical analysis. The method has the advantage of speed and economy, while it also yields a large primary clonal virus stock. HSV clones resistant to phosphonoacetic acid (PAA) and 5-iodo-2'-deoxyuridine (IUdR) could be readily isolated by the described technique.

PubMed Disclaimer

References

    1. Proc Soc Exp Biol Med. 1974 Feb;145(2):690-4 - PubMed
    1. Antimicrob Agents Chemother. 1975 Mar;7(3):289-93 - PubMed
    1. Antimicrob Agents Chemother. 1972 Dec;2(6):476-84 - PubMed
    1. Proc Soc Exp Biol Med. 1975 Feb;148(2):324-8 - PubMed
    1. Ann N Y Acad Sci. 1955 Sep 27;61(4):790-800 - PubMed

Publication types

LinkOut - more resources