[Significance of FCM-DNA ploidy pattern, AgNOR counting, hTERT and PCNA expression in differentiating malignant from benign serous effusion]

Abstract

Background & objective: Differentiating malignant from benign serous effusion is still a difficulty in clinic at present. This study was to explore the clinical value of flow cytometry (FCM)-DNA ploidy, argyrophilic nucleolar organizer region (AgNOR) staining, human telomerase reverse transcriptase (hTERT) and proliferating cell nuclear antigen (PCNA) expression in differentiating malignant from benign serous effusion.

Methods: Sixty-seven patients with serous effusion, treated in People's Hospital of Huaibei City from Jun. 2004 to Jun. 2005, were selected. Besides routine exfoliated cytology examination, FCM-DNA ploidy analysis and AgNOR staining was performed, hTERT and PCNA expression were detected by SP immunocytology.

Results: There were significant differences in FCM-DNA ploidy, AgNOR staining, hTERT and PCNA expression between malignant and benign serous effusion. Compared with exfoliated cytology, the sensitivity of AgNOR staining was the highest (89.1%), the specificity of cytology, hTERT and PCNA expression was the highest (100%), and the accuracy of AgNOR staining and hTERT expression was the highest (89.6%). According to combination examination, the sensitivity was increased to 91.3% through combining cytology with hTERT expression.

Conclusion: DNA aneuploidy, hTERT and PCNA expression and AgNOR counting increase are correlated to malignant serous effusion, and can be important auxiliary measures for serous cytology.