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Comparative Study
. 2007 Apr;45(4):1363-5.
doi: 10.1128/JCM.02430-06. Epub 2007 Feb 14.

Optimization of culture of Leptospira from humans with leptospirosis

Vanaporn Wuthiekanun  1 , Wirongrong Chierakul, Direk Limmathurotsakul, Lee D Smythe, Meegan L Symonds, Michael F Dohnt, Andrew T Slack, Roongreung Limpaiboon, Yupin Suputtamongkol, Nicholas J White, Nicholas P J Day, Sharon J Peacock
Affiliations
Comparative Study

Optimization of culture of Leptospira from humans with leptospirosis

Vanaporn Wuthiekanun et al. J Clin Microbiol. 2007 Apr.

Abstract

A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and clotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.

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References

    1. Doungchawee, G., D. Phulsuksombat, P. Naigowit, Y. Khoaprasert, N. Sangjun, S. Kongtim, and L. Smythe. 2005. Survey of leptospirosis of small mammals in Thailand. Southeast Asian J. Trop. Med. Public Health 36:1516-1522. - PubMed
    1. Ellinghausen, H. C., Jr., and W. G. McCullough. 1965. Nutrition of Leptospira pomoa and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am. J. Vet. Res. 26:45-51. - PubMed
    1. Faine, S., B. Adler, C. Bolin, and P. Perolat (ed.). 1999. Leptospira and leptospirosis, 2nd ed. MediSci, Melbourne, Australia.
    1. Griffith, M. E., L. L. Horvath, W. V. Mika, J. S. Hawley, J. E. Moon, D. R. Hospenthal, and C. K. Murray. 2006. Viability of Leptospira in BacT/ALERT MB media. Diagn. Microbiol. Infect. Dis. 54:263-266. - PubMed
    1. Levett, P. N. 2001. Leptospirosis. Clin. Microbiol. Rev. 14:296-326. - PMC - PubMed

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