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Multicenter Study
. 2007 Nov;135(8):1274-81.
doi: 10.1017/S0950268807008102. Epub 2007 Feb 19.

Relationship of pulsed-field profiles with key phage types of Salmonella enterica serotype Enteritidis in Europe: results of an international multi-centre study

T M Peters  1 , C Berghold, D Brown, J Coia, A M Dionisi, A Echeita, I S T Fisher, A J Gatto, N Gill, J Green, P Gerner-Smidt, M Heck, I Lederer, S Lukinmaa, I Luzzi, C Maguire, R Prager, M Usera, A Siitonen, E J Threlfall, M Torpdahl, H Tschäpe, W Wannet, W K Van der Zwaluw
Affiliations
Multicenter Study

Relationship of pulsed-field profiles with key phage types of Salmonella enterica serotype Enteritidis in Europe: results of an international multi-centre study

T M Peters et al. Epidemiol Infect. 2007 Nov.

Abstract

Salmonella is one of the most common causes of foodborne infection in Europe with Salmonella enterica serovar Enteritidis (S. Enteritidis) being the most commonly identified serovar. The predominant phage type for S. Enteritidis is phage type (PT) 4, although PT 8 has increased in incidence. Within these phage types, pulsed-field gel electrophoresis (PFGE) provides a method of further subdivision. The international project, Salm-gene, was established in 2001 to develop a database of PFGE profiles within nine European countries and to establish criteria for real-time pattern recognition. It uses DNA fingerprints of salmonellas to investigate outbreaks and to evaluate trends and emerging issues of foodborne infection within Europe. The Salm-gene database contains details of about 11 700 S. Enteritidis isolates, demonstrating more than 65 unique PFGE profiles. The clonal nature of S. Enteritidis is evidenced by the high similarity and distribution of PFGE profiles. Over 56% (6603/11 716) of the submitted isolates of several different phage types were profile SENTXB.0001, although this profile is most closely associated with PT 4. The next most common profiles, SENTXB.0002 and SENTXB.0005, were closely associated with PT 8 and PT 21 respectively. Studies to investigate the relationship of profile types with outbreaks and possible vehicles of infection suggest that the incidence of PFGE profile SENTXB.0002, and thus PT 8, in some countries may be due to importation of foods or food production animals from Eastern Europe, where PT 8 is amongst the most frequently identified phage types. Collation of subtyping data, especially in the commonly recognized phage types, is necessary in order to evaluate trends and emerging issues in salmonella infection.

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Figures

Fig. 1
Fig. 1
Relationship between the most common phage types and their pulsed-field gel electrophoresis profiles. ■, PT 4; □, PT 8;formula image, PT 6;formula image, PT 14b;formula image, PT 21;formula image, PT 1.
Fig. 2
Fig. 2
Relationship between PFGE profile SENTXB.0002 and phage types 8 (□) and 14b (■).
Fig. 3
Fig. 3
Relationship between PFGE profile SENTXB.0005 and phage types 21 (□) and 1 (■).
See this image and copyright information in PMC

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