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Review
. 2007 Feb;4(2):e9.
doi: 10.1371/journal.pmed.0040009.

Cell-based bone tissue engineering

Gert J Meijer  1 , Joost D de Bruijn, Ron Koole, Clemens A van Blitterswijk
Affiliations
Review

Cell-based bone tissue engineering

Gert J Meijer et al. PLoS Med. 2007 Feb.

Abstract

The authors review the available data on bone tissue engineering and discuss possible new research areas that could help to make bone tissue engineering a clinical success.

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Conflict of interest statement

Competing Interests: JDB is founder of Progentix BV. CAB is co-founder of IsoTis SA, Progentix BV, and CAM implants BV, which are all active in bone substitution, and has some financial interest in the first two companies. These companies had no role in the decision to submit this paper, or in its preparation. GJM and RK declare that they have no competing interests.

Figures

Figure 1
Figure 1. Alveolar Defect and Reconstruction with Tissue Engineering
Patient (male, 20 years) has lost his upper left incisor during an accident. The alveolar defect was reconstructed with tissue engineering techniques. (A) Radiograph of the significant alveolar defect as a result of the accident. (B) Radiograph of the defect reconstructed with ceramic scaffold (HA) covered with cultured MSCs (white arrow). (C) Radiograph; four months later a dental implant was installed. (D) Intra-oral view; arrow points to the crown, which was fixated on the implant.
Figure 2
Figure 2. Samples and Histology of the Patient Shown in Figure 1
(A) HA particles stained with methylene blue immediately after seeding of the MSCs, showing cell distribution. (B) Idem stained with trypan blue after one week of culturing, showing cell vitality. (C) Histology six weeks after subcutaneous implantation in mice, showing in vivo bone formation (white arrow) in contact with HA particle (black arrow). (D) Histology after four months of implantation in the upper left tooth region, showing bone formation (white arrow) induced by the implanted cells in contact with the HA (black arrow).
See this image and copyright information in PMC

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