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Randomized Controlled Trial
. 2007 Feb 22:6:8.
doi: 10.1186/1475-2840-6-8.

Pro-oxidant effect of alpha-tocopherol in patients with type 2 diabetes after an oral glucose tolerance test--a randomised controlled trial

Mark S Winterbone  1 , Mike J Sampson, Shikha Saha, Jackie C Hughes, David A Hughes
Affiliations
Randomized Controlled Trial

Pro-oxidant effect of alpha-tocopherol in patients with type 2 diabetes after an oral glucose tolerance test--a randomised controlled trial

Mark S Winterbone et al. Cardiovasc Diabetol. .

Abstract

Background: As a part of a larger study investigating the effects of alpha-tocopherol on gene expression in type 2 diabetics we observed a pro-oxidant effect of alpha-tocopherol which we believe may be useful in interpreting outcomes of large intervention trials of alpha-tocopherol.

Methods: 19 type 2 diabetes subjects were randomised into two groups taking either 1200 IU/day of alpha-tocopherol or a matched placebo for 4 weeks. On day 0 and 29 of this study oxidative DNA damage was assessed in mononuclear cells from fasted blood samples and following a 2 h glucose tolerance test (GTT).

Results: On day 0 there was no significant difference in oxidative DNA damage between the two groups or following a GTT. On day 29 there was no significant difference in oxidative DNA damage in fasted blood samples, however following a GTT there was a significant increase in oxidative DNA damage in the alpha-tocopherol treatment group.

Conclusion: High dose supplementation with alpha-tocopherol primes mononuclear cells from patients with type 2 diabetes for a potentially damaging response to acute hyperglycaemia.

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Figures

Figure 1
Figure 1
Change in oxidative DNA damage, assessed by 8-oxoguanine fluorescence, in mononuclear cells isolated from type 2 diabetes patients before (GTT1) and after (GTT2) taking 1200 IU/day α-tocopherol or matched placebo for 4 weeks, following a glucose tolerance test. Data shown are means; error bars represent 95% confidence intervals. AT: α-tocopherol, GTT: Glucose Tolerance Test
See this image and copyright information in PMC

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