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. 2007 May;45(5):1621-3.
doi: 10.1128/JCM.02145-06. Epub 2007 Feb 28.

Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR

Seweryn Bialasiewicz  1 , David M Whiley, Michael D Nissen, Theo P Sloots
Affiliations

Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR

Seweryn Bialasiewicz et al. J Clin Microbiol. 2007 May.

Abstract

Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.

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References

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