doi: 10.1016/0888-7543(92)90404-g.
Walking, cloning, and mapping with yeast artificial chromosomes: a contig encompassing D21S13 and D21S16
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- PMID: 1733862
- DOI: 10.1016/0888-7543(92)90404-g
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Walking, cloning, and mapping with yeast artificial chromosomes: a contig encompassing D21S13 and D21S16
Genomics.
1992 Jan.
Abstract
Chromosome 21 has often been used as a model system for the development of genome mapping and cloning strategies in humans. In this report methods for systematic chromosome walking, cloning, and mapping are exemplified in the construction of a 1.5-Mb yeast artificial chromosome (YAC) contig encompassing and extending 400 kb beyond each of the genetic loci D21S13 and D21S16. Isolation of insert-terminal sequences from YACs in this contig provides a set of closely spaced physical markers. These have been used to generate a long-range genomic restriction map.
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